Fluorescence vs. Fluorescence

• Scatter Plot
• FITC – Annexin V positive show as higher fluorescence on the 525/45nm channel, and express the cell marker for programmed cell death.
• PI positive show as higher fluorescence on the 561nm/LP channel, and express dead or necrotic cells.
• This results in the populations shown below

Histogram

• Healthy: Q4
• Early Stage Apoptosis: Q3
• Late Stage Apoptosis: Q2
• Annexin-/ PI+: Q1