Application: Apoptosis

Fluorescence vs. Fluorescence

  • Scatter Plot
  • FITC – Annexin V positive show as higher fluorescence on the 525/45nm channel, and express the cell marker for programmed cell death.
  • PI positive show as higher fluorescence on the 561nm/LP channel, and express dead or necrotic cells.
  • This results in the populations shown below

Histogram

  • Healthy: Q4
  • Early Stage Apoptosis: Q3
  • Late Stage Apoptosis: Q2
  • Annexin-/ PI+: Q1