Fluorescence vs. Fluorescence
- Scatter Plot
- FITC – Annexin V positive show as higher fluorescence on the 525/45nm channel, and express the cell marker for programmed cell death.
- PI positive show as higher fluorescence on the 561nm/LP channel, and express dead or necrotic cells.
- This results in the populations shown below
- Healthy: Q4
- Early Stage Apoptosis: Q3
- Late Stage Apoptosis: Q2
- Annexin-/ PI+: Q1