Large particles

Brookhuis Particle Deposition Analyzer

  • Information you do not have today: precipitation of particles >15µm – they are in your cleanroom – you just don’t see them today
  • Close to your product: those particles directly impact yield and quality
  • Learn about your processes: data about large particles on surfaces gives more insight in processes than airborne particles alone
  • Ask your questions:


TOC online

Horiba HT110

  • proven :
    UV oxidation
  • reliable:
    gadget free
  • compliant:
    USP<643>,EP2.2.44, JP2.59

  • UV-Oxidation and conductivity determination: This method eliminates the need for reagents and carrier gases. It delivers an accurate measurement at low concentrations, like for instance in WFI (Water For Injection) and reduces the cost of operation at the same time.
  • “Debubbler” embedded: Bubbles in production systems tend to cause unstable readings. The embedded “Debubbler” eliminates the influence of air and guarantees a stable reading.
  • Maintenance cycle: “ONLY ONCE PER YEAR”: Maintenance only consists of annual UV-lamp exchange and annual calibration.

Application: Cell Viability

  • PI positive show as higher fluorescence on the 561/LP channel, and express dead cells.
  • Size and counts are determined highly precise through electric aperture-impedance (Coulter Principle).
  • Viability is shown in the yellow box on top, counts of live and dead cells underneath the scatter plot.

Application: Apoptosis

Fluorescence vs. Fluorescence

  • Scatter Plot
  • FITC – Annexin V positive show as higher fluorescence on the 525/45nm channel, and express the cell marker for programmed cell death.
  • PI positive show as higher fluorescence on the 561nm/LP channel, and express dead or necrotic cells.
  • This results in the populations shown below


  • Healthy: Q4
  • Early Stage Apoptosis: Q3
  • Late Stage Apoptosis: Q2
  • Annexin-/ PI+: Q1